2021 CRISPR Year in Review Stephen Gasior Jan 29th 2022 Presentation Introduction Novel CRISPR–Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives https://www.mdpi.com/1422-0067/22/7/3327/htm CRISPR tool modifies genes precisely by copying RNA into the genome https://www.nature.com/articles/d41586-019-03392-9 Target Genes and Modified Organisms CRISPR Gene Editing Reduces Disease-Causing Protein in Hereditary Transthyretin Amyloidosis https://journals.lww.com/neurotodayonline/Fulltext/2021/10070/CRISPR_Gene_Editing_Reduces_Disease_Causing.3.aspx CRISPR Hits Home in a First-in-Human Study https://www.liebertpub.com/doi/full/10.1089/crispr.2021.29131.mus CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis https://www.nejm.org/doi/full/10.1056/NEJMoa2107454 OMIM Entry https://www.omim.org/entry/105210 He Inherited A Devastating Disease. A CRISPR Gene-Editing Breakthrough Stopped It https://www.npr.org/sections/health-shots/2021/06/26/1009817539/he-inherited-a-devastating-disease-a-crispr-gene-editing-breakthrough-stopped-it ------------------- CRISPR base editing lowers cholesterol in monkeys https://www.nature.com/articles/s41587-021-00975-8 In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels https://www.nature.com/articles/s41587-021-00933-4 In vivo CRISPR base editing of PCSK9 durably lowers cholesterol in primates https://www.nature.com/articles/s41586-021-03534-y PROPROTEIN CONVERTASE, SUBTILISIN/KEXIN-TYPE, 9; PCSK9 https://www.omim.org/entry/607786 Brain-wide Cas9-mediated cleavage of a gene causing familial Alzheimer’s disease alleviates amyloid-related pathologies in mice https://www.nature.com/articles/s41551-021-00759-0 (BEHIND THE PAPER) https://bioengineeringcommunity.nature.com/posts/crispr-throughout-the-brain-brain-wide-genome-editing-via-bbb-crossing-aav-virus-alleviates-amyloid-related-pathologies-in-mice-fea291f8-cf6d-4fed-a5b0-012994528bdf AMYLOID BETA A4 PRECURSOR PROTEIN; APP https://www.omim.org/entry/104760 -------------------- CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank https://www.sciencedirect.com/science/article/pii/S1934590920300199 (not presented but concurrent work) Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope https://www.nature.com/articles/s41467-020-15892-8 Functional correction of CFTR mutations in human airway epithelial cells using adenine base editors https://academic.oup.com/nar/article/49/18/10558/6370254 CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR https://www.omim.org/entry/602421 -------------------- Transient CRISPR-Cas Treatment Can Prevent Reactivation of HIV-1 Replication in a Latently Infected T-Cell Line https://www.mdpi.com/1999-4915/13/12/2461/htm# ----------------------- A Transposon-Associated CRISPR/Cas9 System Specifically Eliminates both Chromosomal and Plasmid-Borne mcr-1 in Escherichia coli https://journals.asm.org/doi/full/10.1128/AAC.01054-21 --------------------------- First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts https://www.mdpi.com/1422-0067/22/1/396/htm CRISPR/Cas9-mediated genome editing in Hevea brasiliensis https://www.sciencedirect.com/science/article/pii/S0926669021001825 Establishment of CRISPR/Cas9 mediated targeted mutagenesis in hop (Humulus lupulus) https://www.sciencedirect.com/science/article/pii/S0981942821000085 Efficient CRISPR/Cas9-mediated genome editing in Rehmannia glutinosa https://link.springer.com/article/10.1007/s00299-021-02723-3 An Efficient Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-Associated Protein 9 Mutagenesis System for Oil Palm (Elaeis guineensis) https://europepmc.org/article/PMC/8647858 CRISPR/Cas9 genome editing in ergot fungus Claviceps purpurea https://www.sciencedirect.com/science/article/pii/S0168165620302649 --------------------------- CRISPR-based knock-out of eIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus https://www.sciencedirect.com/science/article/pii/S0168945221003563 Novel disease resistance gene paralogs created by CRISPR/Cas9 in soy https://link.springer.com/article/10.1007/s00299-021-02678-5 CRISPR/Cas9 mediated mutagenesis of MORE AXILLARY GROWTH 1 in tomato confers resistance to root parasitic weed Phelipanche aegyptiaca https://www.nature.com/articles/s41598-021-82897-8 CRISPR/Cas9-mediated editing of DMR6 orthologue in banana (Musa spp.) confers enhanced resistance to bacterial disease https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.13614 Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes https://www.nature.com/articles/s41598-021-83972-w Characterization of SHATTERPROOF Homoeologs and CRISPR-Cas9-Mediated Genome Editing Enhances Pod-Shattering Resistance in Brassica napus L. https://www.liebertpub.com/doi/full/10.1089/crispr.2020.0129 Biallelic editing of the LOB1 promoter via CRISPR/Cas9 creates canker-resistant ‘Duncan’ grapefruit https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-04-21-0144-R CRISPR/Cas9‐mediated genome editing of MaACO1 (aminocyclopropane‐1‐carboxylate oxidase 1) promotes the shelf life of banana fruit https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051599/ GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response https://academic.oup.com/plphys/article/186/3/1660/6180079 Overexpression of OsRLCK241 confers enhanced salt and drought tolerance in transgenic rice (Oryza sativa L.) https://www.sciencedirect.com/science/article/pii/S0378111920309471 Origin Story CRISPR Gets Its Origin Story https://www.liebertpub.com/doi/full/10.1089/crispr.2021.29137.knl The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases https://www.science.org/doi/10.1126/science.abj6856 Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease https://www.nature.com/articles/s41586-021-04058-1 Cautionary Tale Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing https://www.nature.com/articles/s41588-021-00838-7 [uncited review] CRISPR and Chromothripsis: Proceed with Caution https://www.liebertpub.com/doi/full/10.1089/crispr.2021.29128.sma [uncited review] CRISPR–Cas9 can cause chromothripsis https://www.nature.com/articles/s41588-021-00881-4 CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells https://www.nature.com/articles/s41467-021-25190-6 Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing https://www.nature.com/articles/s41467-021-26097-y Technology Advances Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in mice https://www.nature.com/articles/s41467-021-26714-w Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing https://www.nature.com/articles/s41587-021-01133-w Advances in Agrobacterium transformation and vector design result in high-frequency targeted gene insertion in maize https://onlinelibrary.wiley.com/doi/10.1111/pbi.13613 Meet the CRISPR pioneers who are making gene editing easy https://www.fastcompany.com/90613767/meet-the-crispr-pioneers-who-are-making-gene-editing-easy Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering https://www.nature.com/articles/nbt.3718 https://www.inscripta.com/products/onyx-instrument New Tools CRISPR-Associated Transposase System Can Insert Multiple Copies of Donor DNA into the Same Target Locus https://www.liebertpub.com/doi/full/10.1089/crispr.2021.0019 Find and cut-and-transfer (FiCAT) mammalian genome engineering https://www.nature.com/articles/s41467-021-27183-x [uncited review] BEHIND THE PAPER An Cas9 guided transposon enables a cut and paste shortcut for mammalian genome engineering https://bioengineeringcommunity.nature.com/posts/an-cas9-guided-transposon-enables-a-cut-and-paste-shortcut-for-mammalian-genome-engineering Drag-and-drop genome insertion without DNA cleavage with CRISPRdirected integrases https://europepmc.org/article/ppr/ppr414553 --------------------------------- Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells https://www.nature.com/articles/s41467-021-26469-4 Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing https://www.sciencedirect.com/science/article/pii/S1097276521006481 Programmed genome editing by a miniature CRISPR-Cas12f nuclease https://www.nature.com/articles/s41589-021-00868-6 Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus https://www.nature.com/articles/s41587-021-01009-z ---------------------------------- Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing https://www.sciencedirect.com/science/article/abs/pii/S0092867421003536 ------------------------- A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry https://www.nature.com/articles/s41467-021-21213-4 For further watching pleasure Videos On This Page https://worldcrisprday.com/s/agenda97/home