2022 CRISPR Year in Review Science Circle January 7th 2023 Stephen Gasior, Ph.D. a.k.a. Stephen Xootfly Scientist Corteva Agriscience https://www.sciencecircle.org/event/crispr-3/ Background ---------- Nidhi, Sweta, et al. "Novel CRISPR–Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives." International Journal of Molecular Sciences 22.7 (2021): 3327. https://www.mdpi.com/1422-0067/22/7/3327 Kropocheva, E. V., et al. "Prokaryotic Argonaute Proteins as a Tool for Biotechnology." Molecular Biology (2022): 1-20. https://link.springer.com/article/10.1134/S0026893322060103 Saber Sichani, Ali, et al. "A Review on Advanced CRISPR-Based Genome-Editing Tools: Base Editing and Prime Editing." Molecular Biotechnology (2022): 1-12. https://link.springer.com/article/10.1007/s12033-022-00639-1 Target Genes and Modified Organisms ----------------------------------- Wang, Wenshu, et al. "A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield." The Crop Journal (2022). https://www.sciencedirect.com/science/article/pii/S2214514122000344 Sokka, Joonas, et al. "CRISPR activation enables high-fidelity reprogramming into human pluripotent stem cells." Stem cell reports 17.2 (2022): 413-426. https://www.sciencedirect.com/science/article/pii/S2213671121006561 Foy, Susan P., et al. "Non-viral precision T cell receptor replacement for personalized cell therapy." Nature (2022): 1-3. https://www.nature.com/articles/s41586-022-05531-1 Wang, Li-Bin, et al. "A sustainable mouse karyotype created by programmed chromosome fusion." Science 377.6609 (2022): 967-975. https://www.science.org/doi/abs/10.1126/science.abm1964 (picture source https://www.genengnews.com/topics/omics/sustainable-chromosome-level-engineering-is-a-success-in-mice/) CRISPR in Agriculture: 2022 in Review https://innovativegenomics.org/news/crispr-agriculture-2022/ Therapy Advances ---------------- GOSH patient receives world-first treatment for her 'incurable' T-cell leukaemia https://www.gosh.nhs.uk/news/gosh-patient-receives-world-first-treatment-for-her-incurable-t-cell-leukaemia/ Alyssa's story: Base Editing & CAR T-Cell Therapy at Great Ormond Street Hospital https://youtu.be/x4clNXVVLJw Excision BioTherapeutics Doses First Participant in EBT-101 Phase 1/2 Trial Evaluating EBT-101 as a Potential Cure for HIV September 15, 2022 07:00 ET | Source: Excision BioTherapeutics https://www.globenewswire.com/news-release/2022/09/15/2516733/0/en/Excision-BioTherapeutics-Doses-First-Participant-in-EBT-101-Phase-1-2-Trial-Evaluating-EBT-101-as-a-Potential-Cure-for-HIV.html Ottaviano, Giorgio, et al. "Phase 1 clinical trial of CRISPR-engineered CAR19 universal T cells for treatment of children with refractory B cell leukemia." Science Translational Medicine 14.668 (2022): eabq3010. https://www.science.org/doi/abs/10.1126/scitranslmed.abq3010 CRISPR Therapeutics and ViaCyte, Inc. Announce First Patient Dosed in Phase 1 Clinical Trial of Novel Gene-Edited Cell Replacement Therapy for Treatment of Type 1 Diabetes (T1D) February 2, 2022 https://viacyte.com/press-releases/crispr-therapeutics-and-viacyte-inc-announce-first-patient-dosed-in-phase-1-clinical-trial-of-novel-gene-edited-cell-replacement-therapy-for-treatment-of-type-1-diabetes-t1d/ Edits to a cholesterol gene could stop the biggest killer on earth https://www.technologyreview.com/2022/07/12/1055773/crispr-gene-editing-cholesterol/ First pig-to-human heart transplant: what can scientists learn? https://www.nature.com/articles/d41586-022-00111-9 Will pigs solve the organ crisis? The future of animal-to-human transplants https://www.nature.com/articles/d41586-022-03794-2 Delivery Advances ----------------- Mohanna, Seyedeh Zeinab Mirjalili, et al. "LNP-mediated delivery of CRISPR RNP for wide-spread in vivo genome editing in mouse cornea." Journal of Controlled Release 350 (2022): 401-413. https://www.sciencedirect.com/science/article/pii/S0168365922005491 Han, Jeong Pil, et al. "In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapy." Science advances 8.3 (2022): eabj6901. https://www.science.org/doi/full/10.1126/sciadv.abj6901 Tian, Songhai, et al. "Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms." Cell reports 38.10 (2022): 110476. https://www.sciencedirect.com/science/article/pii/S2211124722002091 Wu, Han, et al. "Cell-penetrating peptide: A powerful delivery tool for DNA-free crop genome editing." Plant Science (2022): 111436.(Review) https://www.sciencedirect.com/science/article/pii/S0168945222002618 Bilichak, Andriy, et al. "Genome editing in wheat microspores and haploid embryos mediated by delivery of ZFN proteins and cell‐penetrating peptide complexes." Plant biotechnology journal 18.5 (2020): 1307-1316. https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.13296 Lee, Seonghyun, et al. "Precision mitochondrial DNA editing with high-fidelity DddA-derived base editors." Nature Biotechnology (2022): 1-9. https://www.nature.com/articles/s41587-022-01486-w Lee, Seonghyun, et al. "Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases." Genome Biology 23.1 (2022): 1-10. https://link.springer.com/article/10.1186/s13059-022-02782-z (Picture from here) Lim, Kayeong, Sung-Ik Cho, and Jin-Soo Kim. "Nuclear and mitochondrial DNA editing in human cells with zinc finger deaminases." Nature communications 13.1 (2022): 1-10. https://www.nature.com/articles/s41467-022-27962-0 Gonzalez, Trevor J., et al. "Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing." Nature Communications 13.1 (2022): 1-17. https://www.nature.com/articles/s41467-022-33745-4 New Tools --------- Yarnall, Matthew TN, et al. "Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases." Nature Biotechnology (2022): 1-13. https://www.nature.com/articles/s41587-022-01527-4 Kuang, Chenzhong, Yan Xiao, and Dirk Hondmann. "Cleavage-free human genome editing." Molecular Therapy 30.1 (2022): 268-282. https://www.sciencedirect.com/science/article/pii/S1525001621006377 Rezazade Bazaz, Mahere, Mohammad M. Ghahramani Seno, and Hesam Dehghani. "Transposase-CRISPR mediated targeted integration (TransCRISTI) in the human genome." Scientific reports 12.1 (2022): 1-18. https://www.nature.com/articles/s41598-022-07158-8 Lisitskaya, Lidiya, et al. "Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity." Nature communications 13.1 (2022): 1-15. https://www.nature.com/articles/s41467-022-32079-5 Tech Advances – RNA targeting ----------------------------- Morelli, Kathryn H., et al. "An RNA-targeting CRISPR–Cas13d system alleviates disease-related phenotypes in Huntington’s disease models." Nature Neuroscience (2022): 1-12. https://www.nature.com/articles/s41593-022-01207-1 Powell, Jackson E., et al. "Targeted gene silencing in the nervous system with CRISPR-Cas13." Science advances 8.3 (2022): eabk2485. https://www.science.org/doi/full/10.1126/sciadv.abk2485 Zheng, Ziwen, et al. "Preventing autosomal-dominant hearing loss in Bth mice with CRISPR/CasRx-based RNA editing." Signal transduction and targeted therapy 7.1 (2022): 1-13. https://www.nature.com/articles/s41392-022-00893-4 New Uses -------- Dhainaut, Maxime, et al. "Spatial CRISPR genomics identifies regulators of the tumor microenvironment." Cell 185.7 (2022): 1223-1239. https://www.sciencedirect.com/science/article/abs/pii/S0092867422001957 Rovira-Clavé, Xavier, et al. "Spatial epitope barcoding reveals clonal tumor patch behaviors." Cancer Cell 40.11 (2022): 1423-1439. https://www.sciencedirect.com/science/article/pii/S1535610822004433 (review for two above: https://www.frontiersin.org/articles/10.3389/fimmu.2022.1041451/full) Hu, Chunyi, et al. "Craspase is a CRISPR RNA-guided, RNA-activated protease." Science 377.6612 (2022): 1278-1285. https://www.science.org/doi/abs/10.1126/science.add5064 Policy News and In the Media ---------------------------- Harrison, Charlotte. "CRISPR beef cattle get FDA green light." (2022): 448-448. https://www.nature.com/articles/s41587-022-01297-z Kenya joins Nigeria in Africa in approving guidelines for CRISPR and other gene editing techniques https://geneticliteracyproject.org/2022/03/18/kenya-joins-nigeria-as-african-countries-approving-guidelines-for-crispr-and-other-gene-editing-techniques Genetic Technology (Precision Breeding) Bill 2022-23 https://commonslibrary.parliament.uk/research-briefings/cbp-9557 https://makepeoplebetterfilm.com/ https://www.nytimes.com/2022/12/14/movies/make-people-better-review.ht